Automation of a single-DNA molecule stretching device
نویسندگان
چکیده
منابع مشابه
Automation of a single-DNA molecule stretching device.
We automate the manipulation of genomic-length DNA in a nanofluidic device based on real-time analysis of fluorescence images. In our protocol, individual molecules are picked from a microchannel and stretched with pN forces using pressure driven flows. The millimeter-long DNA fragments free flowing in micro- and nanofluidics emit low fluorescence and change shape, thus challenging the image an...
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We describe a method for observing real time replication of individual DNA molecules mediated by proteins of the bacteriophage replication system. Linearized lambda DNA is modified to have a biotin on the end of one strand, and a digoxigenin moiety on the other end of the same strand. The biotinylated end is attached to a functionalized glass coverslip and the digoxigeninated end to a small bea...
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Single-molecule studies of the interactions of DNA and proteins are important in a variety of biological or biotechnology processes ranging from the protein’s search for its DNA target site, DNA replication, transcription, or repair, and genome sequencing. A critical requirement for single-molecule studies is the stretching and immobilization of otherwise randomly coiled DNA molecules. Several ...
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We show how to construct nonequilibrium thermodynamics for systems too small to be considered thermodynamically in a traditional sense. Through the use of a nonequilibrium ensemble of many replicas of the system which can be viewed as a large thermodynamic system, we discuss the validity of nonequilibrium thermodynamics relations and analyze the nature of dissipation in small systems through th...
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DNA stretching has been an intensively studied topic due to its involvement in the cellular functions. In this work, we studied DNA stretching based on microfluidics and single particle tracking techniques. Microfluidics generates well-defined flow field within microscale channels and potentially allows the incorporation of chemical and biological assays with the single molecule experiments. Si...
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ژورنال
عنوان ژورنال: Review of Scientific Instruments
سال: 2015
ISSN: 0034-6748,1089-7623
DOI: 10.1063/1.4922068